Title : Synthesis of Carob honey Loaded Chitosan Nanoparticles and its potential Effect on Breast Cancer Cell Line
Abstract:
Embedding natural products into chitosan nanoparticles (CNPs) is an effective way to produce a novel combination with improved biological activities. Therefore, this study aims to synthesize CNPs, incorporate carob honey (CH) into CNPs, and determine the potential effect of the synthesized nanoparticles on MDA-MB-231 breast cancer cell line. The nanoparticles were synthesized using ionic gelation method, MTT and tryphan blue assays were used to study the anti-proliferative effect of the nanoparticles. Successful synthesis and loading of CH in CNPs were confirmed after due characterization. CNPs and carob honey laded chitosan nanoparticles (CHCNPs) have zeta potentials of 18.98 mV and 27.27 mV respectively. The sizes of CNPs and CHCNPs are 86.51 nm and 101.3 nm respectively, and the polydispersity index of CNPs is 0.2393 and, 0.2265 for the CHCNPs. CHCNPs showed a significant (p<0.05) antiproliferative effect against MDA-MB-231 cell line with IC50 of 25±5 μg/mL - 18±2.6 μg/mL in comparison to the free CNPs which has IC50 values of 240 ± 9.2 – 110 ± 18.03 μg/mL within the 24 – 72 hr treatment period. The remarkable antiproliferative effect observed in the CHCNPs may be ascribed to the rich bioactive constituents detected in CH through gas chromatography mass spectrometry analysis. Some of the detected compounds are; Quercetin, Squalene, 5-(Hydroxymethyl) furfural and Tricyclo[4.2.1.1(2,5)]dec-3-ene-9,10-dione. These findings demonstrate the feasibility of loading CH in CNPs to develop a nanoformulation that could potentially serve as a treatment for breast cancer. However, there is a need for further research on the safety, dosage optimization, and mechanisms of action of the nanoparticles.
