Pulmonary arterial hypertension (PAH) is one of the main risk factors for cardiovascular, cerebrovascular and heart failure diseases, which are important causes of mortality in Mexico. In just six years, between 2000 and 2006, HBP prevalence increased 19.7% to one in three Mexican adults (31.6%). An increase in substances that close the arteries (vasoconstrictors) such as endothelin or thromboxane and at the same time a decrease in the arteries (vasodilators), such as prostacyclin and nitric oxide, have been shown. The drugs for the treatment of the PAH have a very high cost (depending on the variations in the market), which is a limiting factor in our country. In Mexico, there are only three drugs FDA approved for HAP: Ilioprost (Ventavis®), Bosentan (Tracleer®), Tadalafil (Cialis®) and one available: Sildenafil (Viagra®), which will be studied. Optimizing the absorption and efficacy of the drug sildenafil is the main objective of this research. Therefore, the purpose of this project is to synthesize and characterize electrospun nanofibers containing fast dissolving sildenafil for the treatment of chronic pulmonary hypertension. To accomplish this, sildenafil citrate was loaded onto poly (vinyl pyrrolidone) nanofibers (PVP). These nanofibers were synthesized through the electrospinning technique. The loading of the drug was performed by mixing it into the polymer solutions. The modified PVP nanofibers were then chemically characterized for the analysis of functional groups, as well as the integrity of the chemical composition of the nanofibers and the binding of the nanofibers and the drug, FTIR and UV were performed for the physicochemical characterization. Regarding thermal stability, DSC and TGA were used. For the analysis of the surface of the nanofibers, average fiber diameter and percentage of membrane porosity, SEM studies were used. Finally, nanofibrous degradation studies and drug release studies were performed. The results will serve as a basis for further studies, evaluating biocompatibility in cell cultures and using experimental models to evaluate the effectiveness of the system in small animals, with the aim of making the data useful for transfer to clinical trials.
Audience take away:
We will discuss electrospun nanofibers as a potential strategy of drug delivery of sildenafil in children. This information can be applied to different drug delivering in children, nanofibers allow an easier administration of the drug when the treatment is chronical and acute, and will help to avoid issues about correct dose administration and psychological fears in kids when they must be treated.