Title : Preparation and evaluation of herbosome of herbal plant of Lawsonia inermis L. for topical application
Abstract:
Background: Lawsonia inermis L. (Lawsone) was reported to contain carbohydrates, proteins, flavonoids and phenolic compounds, alkaloids, terpenoids. The plant has also been reported to have hepatoprotective, anti-inflammatory, antiviral, antifungal and anticancer properties. Lawsone has low bioavailability because it is less soluble in water and it is rapidly eliminated from body.
Objectives: The aim of this study was to prepare and evaluate the herbosome containing lawsone.
Methods: The herbosomes (P1–P4) containing different molar ratios (1:1, 1:2, 2:1 and 2:2) of lawsone and soya lecithin were prepared by the anti-solvent precipitation technique. Further it was evaluated for % yield, particle size analysis, % EE and characterized by FTIR, DSC and SEM. Antifungal activity of herbosome of lawsone was evaluated on Candida albicans (NCIM 3471) fungi by using ketoconazole as standard drug. The optimized herbosome was further proceeded for herbosome gel using different polymers. Then, ex-vivo permeation study in vivo anti-inflammatory activity was carried out on male wistar rats.
Results: The herbosome of lawsone P1 showed better % yield, drug content, particle size and entrapment efficiency as compared to other herbosomes. The Infra-Red (FT-IR) and Differential Scanning Calorimetry (DSC) studies revealed that there was no interaction between the plant drug and phospholipids. SEM data showed that herbosome of lawsone P1 has irregular size vesicles consisting of soya lecithin and it was found to be intercalated in the lipid layer. Antifungal activity of herbosome P1 (1:1) showed the better zone of inhibition as compared to P2 (1:2), drug lawsone and standard drug ketoconazole till 3 days. The ex-vivo permeation study of herbosme gel of lawsone (PG4) through excised rat skin showed 92.91% of drug permeation up to 6 h. The in vivo anti-inflammatory activity of gel of herbosome of lawsone showed significant anti-inflammatory activity as compared to gel of drug lawsone at 4 h (P<0.001).
Conclusion: Furthermore, Herbosome of Lawsone has shown the potent ex-vivo permeation study and significant in vivo anti-inflammatory activity as compared to plant drug against microbial infection (C. albicans). This may conclude that it have the effective potential to treat skin infections.
Keywords: Herbosome, Lawsone, Soya lecithin, Topical Application.
