Title : Novel 2, 5-disubstituted oxadiazole scaffolds act as potential antitumour and antibacterial agents
Abstract:
Introdution: 1, 3, 4-oxadiazole is a five membered heterocyclic aromatic compound containing two nitrogen atom at position three and four and one oxygen atom present at position one. 1, 3, 4 oxadiazole is thermally stable than other oxadiazoles, these oxadiazole are very important compound in medicinal chemistry due to their biological activities.
Aim and objectives: The main aim and objective of the present research work is the synthesis, characterization and evaluation of antitumour and antibacterial potentiality of 2, 5-disubstituted 1, 3, 4 - Oxadiazole derivatives against EAC cells and HT 29 cell lines and bacterial 3G7E DNA gyrase.
Methodology: The target compounds were synthesized by reflux condensation method and were characterized by modern analytical techniques such as FTIR, H1-NMR and mass spectrometry. The toxicity of synthesized compounds was evaluated by OECD guide lines. In-silico studies were done by AUTO-DOCK programe against targeted proteins bacterial DNA gyrase. In vivo antitumor activity of synthesized compounds (AB1-AB8) on EAC mouse tumour models. Study was carried out with EAC cell line induced malignant ascites in mouse models and in vitro anti-bacterial activity was carried out by agar diffusion method.
Results and discussion: In vivo antitumour activity against EAC mouse tumour models and HT 29 implanted mouse models showed that compound AB3, AB6, AB7 and AB8 had the potential ability to inhibit the growth and proliferation of cancer or tumour cells in comparison to other derivatives. The molecular docking studies showed sufficient interaction with the targeted proteins bacterial DNA gyrase in respect to standard drugs ciprofloxacin to show respective biological activity. However different interaction was observed for different compounds. In vitro antibacterial assay, among these eight synthesized oxadiazole derivatives, compound AB1; AB2 and AB7 were found to be very good antibacterial potentiality with an MIC range of 13-12 µg/ml.
Conclusion: From the present experimental discussion I was concluded that in vivo antitumour activity against EAC mouse tumour models and HT 29 implanted mouse models showed that compound AB3, AB6, AB7 and AB8 had the potential ability to inhibit the growth and proliferation and AB1; AB2 and AB7 were found to be very good antibacterial as well as antifungal potentiality with an MIC range of 13-12 µg/ml; 7-10 µg/ml.
Keywords: Antitumour; antibacterial, molecular docking; EAC etc.
