Abstract:
Hepatitis B, a viral infection that can cause both acute and chronic hepatitis, cirrhosis and hepatocellular carcinoma, is a major global health issue. Hepatocellular carcinoma is one of the most common types of cancer, accounting for roughly 90% of all primary liver tumours. The virus replication is through reverse transcription and most antiviral drugs are nucleoside/nucleotide analogues. Clevudine, a nucleoside analogue licenced in Korea in 2006 has been found effective in controlling HBV infection. Interaction between a dug and the plasma protein is of pharmacological significance as this interaction could affect drug absorption, distribution, metabolism and excretion in the body as well as the physiological activity of the protein. Understanding such interactions offer information about drug-drug interaction, and drug-protein resistance and is helpful to predict potential drug dosages and adverse effects. Serum albumin, a 585 residue-long protein encompassing three domains, I, II and III with their divisions into two subdomains, A and B, is the main drug transporter in blood circulation. It possesses two well-defined drug-binding sites, namely, Site I (subdomain IIA) and Site II (subdomain IIIA). This talk will highlight the interaction results between clevudine and human serum albumin using a variety of spectroscopic techniques i.e., absorption, fluorescence, circular dichroism and atomic force microscopy along with molecular docking.
Biography:
Dr. Saad Tayyab is currently working as a Professor of Pharmaceutical Chemistry in the Faculty of Pharmaceutical Sciences at UCSI University, Kuala Lumpur, Malaysia. Before joining UCSI University in June 2022, Dr. Saad served the University of Malaya (2004-2022) as a Professor of Biochemistry at the Institute of Biological Sciences of the Faculty of Science. He received his B.Sc. (Honors) in Chemistry (1979), M.Sc. in Biochemistry (1981), M.Phil. in Biochemistry (1983) and Ph.D. in Biochemistry (1987), all degrees from the Aligarh Muslim University, India. Earlier, he served the University of Kashmir, India, as a Lecturer in Biochemistry (1987-1988), the Aligarh Muslim University, India, as a Lecturer in Biochemistry/Biotechnology (1988-1992), Lecturer-Senior Grade in Biotechnology (1992-1997), Reader in Biotechnology (1997-2001) and Alemaya University, Ethiopia, as Associate Professor in Biochemistry (2001-2004) under World Bank (ARTP) Project. He was admitted as a Fellow of the Royal Society of Chemistry (FRSC), UK, in December 2017 and a Fellow of the Royal Society of Biology (FRSB), UK, in July 2019. He has been a member of the American Chemical Society since January 2020 and a Life member of the Malaysian Society for Biochemistry and Molecular Biology. Earlier, he received the Wood/Whelan Fellowship from IUBMB and ICSU for pursuing higher training at the Medical College of Georgia, Augusta, USA, during April/May 1994. Diagrams from his publications have been chosen for the journal cover page of Advances in Protein Chemistry and Structural Biology (2021) and Biopolymers (2020). One of his articles was selected in the 10th Anniversary Collection on Molecular Modelling of RSC Advances (2021).
Dr. Saad is currently serving as the Editorial Board Member of Frontiers in Molecular Biosciences (since 2022), Protein and Peptide Letters (since 2019), Letters in Drug Design and Discovery (since 2015) and Biochemistry Research International (since 2013). He has also served as the Chief Editor of the Malaysian Journal of Biochemistry and Molecular Biology (2015-2017). Dr. Saad has published over 150 journal papers, 17 popular articles, 1 book, 1 book chapter, 1 learning aid and 3 book reviews. Several research students (16 Ph.D., 6 M.Phil., 1 M.D. and 13 M.Sc.) completed their degrees under his supervision, and he guided 32 undergraduate projects. His research interest includes drug-protein interaction, protein folding, protein/enzyme stability and protein structure-function. He possesses an h-index of 26, and his name is included in the reviewers’ list of many international journals.
