Title : Chemo-enzymatic approach to synthesis of modified nucleosides
Abstract:
Over two decades, a large number of nucleosides have been synthesized, which demonstrated potent antiviral and antitumor activities and have become cornerstones of treatment for patients with cancer or viral infections. Oligonucleotide-based antisense strategies represent a unique paradigm for the treatment of a wide variety of human diseases. In order to discover a new class of nucleoside derivatives with enhanced biological activities, modifications in the sugar moietiy have been attempted, which provide a remarkable level of control over nucleoside sugar puckering and its biological activity. Herein, we report; (a) the selective biocatalytic acetylation studies on modified 3′-azido-4′-C-hydroxymethylated sugar derivatives with an aim to develop an efficient and easy method for the synthesis of ribo-azido/amino LNA monomers and xylo-azido/amino spiro-oxetano nucleosides and (b) the selective biocatalytic deacetylation studies on modified 3′-azido-4′-C-acetoxymethylated sugar derivatives with an aim to develop an efficient and easy method for the synthesis of ribo-azido/amino spiro-oxetano nucleosides and xylo-azido/amino LNA monomers.
Audience Take Away Notes:
- During the presentation I will explain the use of lipases in academics and industry, apart from this I will also explain novel and greener methodologies for the synthesis of modified nucleosides. As I know that nucleosides are a valuable component of nucleic acid and it shows different types of activities such as antiviral, anticancer, etc.
