Title : Aryl Amine content determination in different Indian brands of hair dyes by analytical techniques
Abstract:
Paraphenylenediamine (PPD) is a chemical substance that is used in hair dye because it is a permanent dye that gives a natural look, and the dyed hair can also be shampooed or permed without losing its colour. PPD-containing hair dyes have been associated with cancer and mutagenicity. Apart from that, PPD has potential toxicity which includes acute toxicity such as allergic contact dermatitis and subacute toxicity. The European Union Cosmetic Directive allows a maximum concentration of 6% PPD in hair dyes, while the Bureau of Indian Standards has set a maximum permissible limit of 30% and not less than 3% PPD after dilution of the powder form and a maximum of 4% and not less than 1.2% after dilution of the oxidation liquid type. A simple rapid,precise,and accurate method has been developed to determine the concentration of para-phenylenediamine in different marketed hair dyes by UV-Visible Spectrophotometer and RP-HPLC. The PPD analysis was performed using NaOH solution as Diluent at 246 nm. The methods were linear in the concentration range from 0-15µg/ml. All the validation parameters were performed according to ICH (Q2)R1 guidelines and all the parameters were found to be within the limits. Analysis of PPD in different brands of hair dyes was performed and all the brands contain PPD within the limits. Hence the developed method can be used for routine analysis of para-phenylenediamine in hair dyes.
