Title : Formulating and assessing spray-dried powder derived from trigonella foenum-graecum seeds for suspending properties in the creation of rivaroxaban nanosuspension
Abstract:
The present study aimed to formulate rivaroxaban nanosuspension by nanoprecipitation with the ultrasonication method and was stabilized by different concentrations of Fenugreek Seed Mucilage, Microcrystalline Cellulose and Carboxymethyl Cellulose. The formulated nanosuspensions were evaluated for saturation solubility studies, particle size, drug content, zeta potential, and in vitro drug release. In the FT-IR spectra of the drug in the physical mixture, it was noted that the peaks had remained unaltered. DSC thermograms of Rivaroxaban and the physical mixture indicated the absence of interaction and the presence of the drug in an unchanged form. The evaluation tests were conducted and optimization was done using Minitab software. Optimized formulation F3 demonstrated an impressive saturation solubility of 0.89 mg/mL. The particle size distribution studies revealed that the optimized formulation F3 showed 79 nm. Formulation F3 exhibited drug content reaching 75%. The zeta potential was measured at -33 mV for formulation F3. Pure drug RN exhibited a cumulative drug release of 20%, and formulation F3 showed 71% cumulative drug release at the end of the 60th min. Rivaroxaban nanosuspension showed a prolonged duration of drug action, enhanced the rate and extent of drug absorption, and ultimately increased the therapy's effectiveness
Audience Take Away Notes:
- The audience will learn how to formulate rivaroxaban nanosuspension using Fenugreek Seed Mucilage, Microcrystalline Cellulose, and Carboxymethyl Cellulose as stabilizers. They will gain insights into assessing key parameters like saturation solubility, particle size, drug content, zeta potential, and in vitro drug release. Utilizing optimization techniques with Minitab software, they can achieve an impressive drug solubility of 0.89 mg/mL, particle size distribution of 79 nm, and 75% drug content in the optimized formulation. This knowledge enables them to design nanosuspensions with prolonged drug action, improved absorption rates, and enhanced therapeutic effectiveness for rivaroxaban administration
- This study provides valuable insights for researchers and pharmaceutical professionals involved in formulating and optimizing nanosuspensions for drug delivery. By employing nanoprecipitation with ultrasonication and stabilizing agents like Fenugreek Seed Mucilage, Microcrystalline Cellulose, and Carboxymethyl Cellulose, they can enhance the solubility and stability of rivaroxaban. The comprehensive evaluation of key parameters such as saturation solubility, particle size, drug content, and in vitro drug release assists in fine-tuning formulations for optimal performance. Utilizing techniques like FT-IR and DSC ensures the integrity of the drug during formulation. Optimization using software like Minitab streamlines the process, enabling the development of nanosuspensions with improved drug release profiles, absorption rates, and therapeutic efficacy, enhancing patient outcomes
- This research provides a valuable foundation for other faculty members to expand their research or integrate findings into teaching materials. The methodology employed, including nanoprecipitation with ultrasonication and the use of various stabilizing agents, offers a reproducible framework for formulating nanosuspensions. Additionally, the comprehensive evaluation of key parameters such as saturation solubility, particle size, drug content, and in vitro drug release provides a basis for further investigation and comparison with other drug delivery systems. Moreover, the utilization of analytical techniques like FT-IR and DSC, along with optimization using Minitab software, demonstrates good scientific practices that can be applied in various research endeavors. Overall, this study serves as a valuable resource for both research and teaching in the field of pharmaceutical sciences
- This research offers a practical solution that could significantly simplify and enhance the efficiency of a designer's job in pharmaceutical formulation. By formulating rivaroxaban nanosuspension through nanoprecipitation with ultrasonication and stabilizing it with Fenugreek Seed Mucilage, Microcrystalline Cellulose, and Carboxymethyl Cellulose, the study provides a systematic approach to overcome challenges related to drug solubility and stability. The comprehensive evaluation of key parameters such as saturation solubility, particle size, drug content, zeta potential, and in vitro drug release enables designers to optimize formulations effectively. Furthermore, the use of analytical techniques like FT-IR and DSC, coupled with optimization through Minitab software, streamlines the formulation process and ensures the integrity of the drug. The resulting nanosuspension exhibits enhanced drug action, absorption rates, and therapeutic effectiveness, offering designers a practical solution to improve drug delivery systems
- This study will significantly improve the accuracy of a design and provide new information to assist in addressing design problems related to pharmaceutical formulations. By formulating rivaroxaban nanosuspension through nanoprecipitation with the ultrasonication method and stabilizing it with different concentrations of Fenugreek Seed Mucilage, Microcrystalline Cellulose, and Carboxymethyl Cellulose, the research offers a systematic approach to enhance drug solubility and stability. The evaluation of key parameters such as saturation solubility, particle size, drug content, zeta potential, and in vitro drug release provides precise data to guide formulation design decisions. Additionally, the utilization of analytical techniques like FT-IR and DSC ensures the integrity of the drug and confirms the absence of interactions, contributing to the accuracy of the design. Furthermore, the optimization process using Minitab software helps in fine-tuning formulations for optimal performance. Overall, the findings from this study offer valuable insights and data that can significantly enhance the accuracy and effectiveness of pharmaceutical formulation designs
List all other benefits:
- Enhanced Drug Solubility: The formulation achieved an impressive saturation solubility of 0.89 mg/mL, which is beneficial for drugs with low aqueous solubility like rivaroxaban
- Optimal Particle Size: The optimized formulation F3 exhibited a particle size of 79 nm, which is favorable for nanosuspensions as it enhances drug delivery and bioavailability
- High Drug Content: Formulation F3 demonstrated a drug content reaching 75%, indicating efficient encapsulation of rivaroxaban within the nanosuspension, maximizing therapeutic efficacy
- Controlled Zeta Potential: The measured zeta potential of -33 mV for formulation F3 suggests good stability of the nanosuspension, preventing particle aggregation and ensuring long-term shelf stability
- Improved Drug Release: Compared to pure drug RN, formulation F3 showed a significantly higher cumulative drug release of 71% at the end of the 60th min, indicating enhanced drug release kinetics and potentially improving patient compliance
- Prolonged Drug Action: The rivaroxaban nanosuspension exhibited a prolonged duration of drug action, suggesting sustained release characteristics that could lead to improved treatment outcomes and reduced dosing frequency
These benefits collectively contribute to the overall efficacy, stability, and patient acceptability of the formulated rivaroxaban nanosuspension
