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Speaker for Pharmaceutical Conferences - Andrey Belousov
Andrey Belousov
Laboratory of Applied Nanotechnology of Belousova, Ukraine
Title : Innovative method of nanotechnology to increase the storage time of RBCs due by stabilizing the molecular structure of proteins and lipids of erythrocyte membranes

Abstract:

This study was devoted to the learning changes in the structure of erythrocyte membranes at the level of molecular bonds during their storage at a positive temperature by means method of infrared spectroscopy (IR). Objects of research were red blood cells (RBCs) into bags containing preservative CPD and RBCs into bags containing preservative CPDA-1. As membrane protective used saline which had previously been treated with magnetite nanoparticles (ICNB) by the Belousov's method. The physiological solution that was treated with nanoparticles was added to the preserved RBCs according to the developed method. Sample of control was the addition of intact saline. Analysis of changes occurring in the IR spectra of samples of control and test in the CPD medium was showed that during the first 28 days storage of: 1. In the suspension of control of the RBCs, noticeable destructive changes in the molecular structure membrane of erythrocytes at the 14th day storage begins. After three weeks, the molecular structure membrane of erythrocytes is completely destroyed; 2. In the sample of test there was a weakening and rupture of molecular bonds only at the 28th day storage of RBCs. Complete destruction of the structure of membrane of erythrocytes occurs at the 35th day storage. Analysis of changes occurring in the IR spectra of samples of control and test in the CPDA-1 medium was showed that during 49 days storage of: 1. In the suspension of control of RBCs noticeable destructive changes in the molecular structure begins in four weeks, and after six weeks storage the molecular structure of erythrocytes membrane are completely destroyed; 2. In the sample of test, a significant weakening of intra-and intermolecular bonds in the structure of erythrocytes membrane occurs after six weeks. However, the complete destruction of the structure is not observed. After seven weeks storage of erythrocytes obvious violations of the molecular structure of lipids and proteins that make up the RBCs are visible but some of the strongest compounds still remain. In general, the results clearly showed that the presented method of application of nanotechnology significantly increases the storage time of RBCs in different versions of preservatives due to mechanisms to reduce violations of the molecular structure of proteins and lipids in the erythrocyte membranes. Presented method of application of nanotechnology is not only safe for use in practice in the Blood Service, Transfusiology and Hematology, but also is the most promising innovation project.

Biography:

Prof. Andrey Nikolaevych Belousov is Doctor of Medicine degree on speciality - Anesthesiology and Intensive Care. Author a new medicine products – nanotechnology preparations based on magnetite nanoparticles (Fe3O4) (www.nanolab.com.ua): Micromage-B (officially registration in Ukraine); Magnet-controlled sorbent brand of MCS-B for extracorporeal detoxication of biological liquids (officially registration in Ukraine and was allowed for medical practice); NanoBiocorrector for intravenous application – ICNB (intracorporal nanosorbent). A.N. Belousov is author new method of extracorporeal hemocorrection using magnet-controlled sorbent (MCS-B). The published more 240 scientific works on results application of nanotechnology preparation in experimental and practical medicine. At now Andrey Belousov - the Head of Laboratory Applied Nanotechnologies in Ukraine, DM, Professor of Department Anesthesiology, Intensive Care, Transfusiology and Hematology Kharkov Medical Academy of Postgraduate Education.

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