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Title: Study of the capability of Chitosan-Glutathione nanoparticles to modulate redox state in human Chondrocytes

Roberto Diaz-Torres

National University of Mexico, Mexico

Biography

Roberto Diaz-Torres, born in Mexico City, Bachelor in pharmaceutical chemistry, he worked about 10 year in the industry (Procter and Gamble, Roche and 3M), MS in physical chemistry, Dr in chemistry specifically in nanomedicine. Researcher since 2008 in the field of drug delivery systems and professor at the National University of Mexico since 2002.

Abstract

The use of nanometric systems as disposal of biologically active substances is a successful tool in different areas of knowledge. In this research we studied nanometric systems with antioxidant capacity to modulate events associated with the redox state in human chondrocytes. We use nanoparticles (NPs) prepared with chitosan and glutathione (GSH), using as an in vitro model a primary culture of human chondrocytes extracted from hyaline cartilage. The cells were exposed to CdCl2 in the presence or not of NPs. CdCl2 is a widely known oxidizing agent. Fluorescence and confocal microscopy showed the location of the NPs within the cells. The results obtained showed that NPs did not affect cell viability significantly. We studied the antioxidant capacity of the NPs by estimating the content of GSH, TBARs, Cell Rox and the enzymatic activity of glutathione peroxidase (GPx). In vitro assays showed that glutathione (GSH) levels, as well as GPx activity and reactive oxygen species (Cell Rox), were modified with both concentrations of NPs; while lipoperoxidation (TBARs) decreased when cells exposed to CdCl2 were in contact with the NPs. All these results suggest the ability of NPs to modulate the cell redox state in a dose-related response.
Audience take away:
• The audience will have the information to know how these nanoparticles act over chondrocytes as therapy agents.   
• This is a very good alternative to modulate the redox balance in joints. 
• This presentation shows the methodologies to evaluate the effect of a toxic agent and how the nanoparticles improve the redox balance.
• Provides new information in the uses of polymeric nanoparticles.