Title : Hollow Mesoporous Silica as Potential Platform for Anti-cancer Drug Delivery in Cancer Treatment
Over the last several years, mesoporous silica nanoparticles (MSNs) have been extensively developed in the field of biomedicine as nanocarriers for delivering anti-cancer drugs, due to their biocompatibility and ease of surface functionalization. However, it has certain limitations for being applied in pharmaceuticals, for example this type of material usually has low drug loading capacity. In this study, hollow mesoporous silica nanoparticles (HMSNs) were fabricated in order to increase the drug loading capacity of nanosilica materials. The synthesized HMSNs possessed inner hollow cores that could remarkably raise the total pore volume and thus improve the capacity for cargo loading. HMSNs were synthesized according to the hard-template method with three main steps: (1) forming of solid SiO2 nanoparticles as templates, (2) forming of core-shell structure by coating MSN layers onto the templates, and (3) forming of hollow core structure by etching away the solid template. The HMSNs product was characterized by TEM, XRD, TGA, FTIR and BET. In addition, drug loading capacity of the material was evaluated with doxorubicin (DOX) as model drug. The results indicated remarkable improvement in drug loading capacity, compared to MSN sample. More importantly, MTT assay data showed that the prepared HMSNs were biocompatible nanocarriers and furthermore, the incorporation of DOX into these HMSNs has been proven successful in reducing the toxicity of DOX. In the interest of the system’s biodistribution, the imaging and measurement on mice with tumor cell lines were conducted and the obtained results indicated excellent accumulation of the desired materials at tumor sites. As a part of histological investigation on HMSNs nano-drug-carrier system, long-term toxicity study was also managed by H&E organ staining. Base on the condition of the cells’ components, for examples cellular shrinkage in liver cells, condensation of chromatin or rupture of cell membrane, etc., the conclusion about toxic features from the administration of HMSNs system can be made, which in this report, showed minor in vivo toxicity. These results demonstrated the potential of HMSNs in the delivery of anticancer agents.